Published on December 2009 | Microbiology

Detection of virulence gene in Aeromonas hydrophila isolated from fish samples using PCR technique

The detection of virulence factors of Aeromonas hydrophila is a key component in determining potential pathogenicity because these factors act multifunctionally and multifactorially. In this study fish samples were collected from local market, Chennai, Tamil Nadu, India and totally 15 fish samples were collected, among these only 7 samples namely, Parai, Kadamba, Kelangan, Shankara, Nethili, Prawn and Suthubhai were found to be positive for Aeromonas hydrophila. The modified Rimler-Shotts medium and Kaper’s multitest medium were used as a selective presumptive isolation medium. For rapid detection of two virulence factors of isolated Aeromonas hydrophila, a polymerase chain reaction assay was used. The detected virulence factors include aerolysin (aer A) and haemolysin (hyl H). The band appearance in the amplified virulence genes of screened fish samples shows the molecular weight of aerolysin (aer A-416bp) and haemolysin (hyl H-597bp).