Published on August 2014 | Nanotechnology, Silver nano particles, Seaweeds, Cancer

In vitro cytotoxic activity of silver nano particle bio synthesized from Colpomenia sinuosa and Halymenia poryphyroides using DLA and EAC cell lines

This study was conducted to investigate the in-vitro cytotoxic activity of silver nanoparticles biosynthesized from Colpomenia sinuosa and Halymenia poryphyroides using DLA and EAC cell lines by trypan blue dye exclusion technique and MTT assay using Mouse L929 cell lines (Lungs fibroblast). The results of the trypan blue dye exclusion assay indicate that the silver nanoparticles biosynthesized from Colpomenia sinuosa and Halymenia poryphyroides inhibits the growth of DLA and EAC cell lines in a dose-dependent manner against the standard drug Curcumin where the silver nanoparticle biosynthesized from Colpomenia sinuosa showed 61.57 % and silver nanoparticle biosynthesized from Halymenia poryphyroides showed 89.36 % in the DLA cell line similarly the silver nanoparticle biosynthesized from Colpomenia sinuosa showed 81.96 % and silver nanoparticle biosynthesized from Halymenia poryphyroides 91.45 % in the EAC cell line. The results of the MTT assay indicated the silver nanoparticles biosynthesized from Colpomenia sinuosa and Halymenia poryphyroides significantly inhibited the proliferation of L929 cells in a dose-dependent manner where the silver nanoparticle biosynthesized from Colpomenia sinuosa showed 37.06 % and silver nanoparticle biosynthesized from Halymenia poryphyroides showed 100 % against the standard drug Curcumin.