Published on April 2009 | Plant Biotechnology

TLC Method for the Determination of Plumbagin in Hairy Root Culture of Plumbagorosea L.

Multiple shoot formation from the medicinal plant Plumbago rosea Linn. was induced on Shoots from nodal segments on Murashige and Skoog media containing cytokinin. BAP (2.0 mg l) added to the media gave1 best Shoot induction, while Kin (2 mg l)induced shoot formation. Four bacterial strains, A4, TR105, LBA 94021 and15834 and the one explant type, in vitro developed shoots were examined for hairy root induction. Among theAgrobacterium rhizogenes strains used, 15834 strain was observed to be the most virulent one.The establishment of hairy root cultures leading to the production of a bioactive compound, Plumbagin and it was confirmed by using TLC-densitometric method.