Published on April 2014 | Microbiology, Nanotechnology, Bacteriology, Seaweeds, Silver nanoparticles
In this study biosynthesis of colloidal silver (Ag) nanoparticles from marine alga, Halymenia poryphyroides were studied against Gram-positive and Gram-negative bacteria. Green synthesis of silver nanoparticles that have environmentally acceptable solvent systems and eco-friendly reducing agents is of great importance. The efficacy was performed using Kirby Bauer Method and the extracellular mechanism of silver nanoparticle creation was characterized by UV-vis spectroscopy, Fourier Transform Infrared (FT-IR) spectroscopy, and X-ray Diffraction (XRD). Particle size distribution and morphology were investigated by Scanning Electron Microscope which showed silver nanoparticles in the size range of 34-80 nm. Fourier transform infrared spectroscopy revealed the possible involvement of reductive groups on the surfaces of silver nanoparticles. Silver nanoparticles showed greater efficacy towards Gram-positive and Gram-negative bacteria compared to the standard antibiotics.