Published on June 2020 | Nanotechnology, Silver nano particles, fungi, Mycology, Seaweeds

BIOSYNTHESIS AND CHARACTERIZATION OF SILVER NANOPARTICLES FROM MARINE MACROSCOPIC RED SEAWEED HALYMENIA PORPHYROIDES BOERGESEN (CRYPTON) AND ITS ANTIFUNGAL EFFICACY AGAINST DERMATOPHYTIC AND NON-DERMATOPHYTIC FUNGI

Objective: The current study illustrates the biosynthesis of economically scalable and energy efficient colloidal silver nano-particles (AgNPs) from marine red seaweed Halymenia porphyroides Boergesen (Crypton) collected from Southeast coast of Tamil Nadu, India, and their anti-fungal efficacy against dermatophytic and non-dermatophytic fungi was evaluated. Methods: The biosynthesis of silver nanoparticles from marine macroscopic red seaweed H. porphyroides Boergesen were synthesized by green synthesis method and characterized by UV–Vis spectroscopy, Fourier transform infrared (FT-IR) spectroscopy, X-ray diffraction, thermogravimetric analysis (TGA), scanning electron microscope (SEM), and transmission electron microscopy (TEM). The efficacy of silver nano-particles against dermatophytic and non-dermatophytic fungi was performed by disk diffusion method. Results: The presence of silver nano-particles with an average size between 34.3 and 80 nm and exhibiting face-centered cubic structure was confirmed. SEM revealed the morphology of the nano-particles as spherical and TEM exhibited the nano-particle distribution. The FT-IR spectra confirmed the presence of potential bio-molecules in the seaweed crude extract which is responsible for reducing and capping the bio-reduced silver nano-particles. The UV absorption spectra at 430.5 nm revealed the characteristic spectra of the silver nano-particles. The purity and the thermal stability of silver nano-particles were revealed by TGA. Silver nano-particles showed significant efficacy against dermatophytes and Rhizopus microsporus among nondermatophytes. Conclusion: Intermediate efficacy was observed against dermatophytes and among non-dermatophytic fungi R. microsporus exhibited better efficacy, whereas Aspergillus flavus were resistant to the bio-synthesized silver nano-particle.