Published on June 2020 | Nanotechnology, Silver nano particles, fungi, Mycology, Seaweeds

Biosynthesis and Characterization of Silver Nanoparticles from Marine Seaweed Colpomenia Sinuosa and its Antifungal Efficacy

The present study illustrates the biosynthesis of colloidal silver nano-particles (AgNPs) from marine seaweed Colpomenia sinuosa and their anti-fungal efficacy was determined against dermatophytic and non-dermatophytic fungi. The dermatophytic fungi used in this study are Microsporum nanum (ATCC 28951) and Trichophyton mentagrophytes (ATCC 28185) whereas the non dermatophytic fungi Aspergillus flavus (ATCC 20048) and Rhizopus microsporus (ATCC 22960). The rich content of phytochemicals, bioactive compounds and secondary metabolites in marine seaweed Colpomenia sinuosa possess the reducing/capping agents that may be environmentally acceptable and eco-friendly for the biosynthesis of silver nano-particles. The efficacy of bio-synthesized silver nano-particles from marine macroscopic brown alga was performed using Kirby Bauer Method and the silver nano-particles bio-synthesized was characterized by UV-vis spectroscopy, Fourier Transform Infrared (FTIR) Spectroscopy, X-ray Diffraction (XRD) and Thermo gravimetric analysis. Particle size distribution and morphology were investigated by scanning electron microscope which showed silver nano-particles in the size range of 54-85 nm. The average size of the silver nano-particle indicated by TEM analysis was found to be 34 nm. The anti-fungal efficacy of silver nano-particles at the concentration 30 μg/mL revealed greater efficacy in dermatophytic fungi while Rhizopus microsporus as non dermatophytic fungus showed better anti-fungal activity when compared to the standard fungal antibiotics used.